22 Biology -- Monera

Differentiate between gram positive bacteria and gram negative bacteria.

Differentiate between gram positive bacteria and gram negative bacteria.

On the basis of the cell wall structure and stain ability with the Gram's stain, bacteria are classified into two groups: Gram positive and Gram negative.

Gram positive bacteria

Gram negative bacteria

The bacteria which retain blue color of crystal violet dye after decolorizing by alcohol are called Gram positive bacteria.
The bacteria which lose blue color of crystal violet dye after decolorizing of alcohol are known as Gram negative bacteria.
1. Cell wall is 25-30 nm thick and has 1 layer. 1. Cell wall is 10-15 nm thick and has 3
2. Peptidoglycan (murein) occupies 20-80% of dry weight of cell wall. 2. Peptidoglycan (murein) constitutes 10-20% of dry weight of cell wall.
3. Retains the  Gram's stain, i.e. show purple or dark violet color. 3. Lose gram's stain. They can be stained with safranin.
4. Very little lipid contents ( 0-2%). 4. High lipid content (10-20%).
5. Lipopolysaccharide layer is absent. 5. Lipopolysaccharide layer is present.
6. susceptible to antibiotics( lysosomes). 6. Due to presence of high lipid content, it is resistant to lysosomes.

7.Spore-forming bacilli, many cocci.          

Example: Staphylococcus, Streptococcus, Mycobacterium, etc.   

7. Most non spore-forming bacilli, spirals and some cocci.

Example: Rhizobium, Vibrio, Salmonella, etc.


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