Introduction to Bio-technology
Introduction to Bio-technologyBiotechnology is a new discipline of science in which the latest technology has been applied to biological organisms for the welfare of society. It is the application ...
REQUIREMENTS FOR TISSUE CULTURE
1. A tissue culture laboratory for:
- Nutrient media preparation, sterilization, cleaning and storage of supplies
- Aseptic condition for working with living cultures
- A controlled environmental condition for growth and developments of culture
- Easy observation and evaluation of culture
2. Nutrients Media composition and preparation to provide:
- The nutrients for the survival of the plant cells, tissues and organs under culture and
- The optimal physical condition of pH, osmotic pressure, etc.
a. Inorganic salts: The major elements are N, P, K, S, Mg and Ca. Other nutrients such as Co, Fe, B, Zn, Mo, Cu, I are microelements.
- Nitrogen: Plays a vital role in growth and differentiation of cultured tissues supplied in the form of NH, along with NO3.
- Phosphorus: Primary buffering constituent supplied in the form of phosphates.
- Sulphur is provided in the form of sulfates, amino acids like L-cysteine, L-methionine and glutathione are satisfactory sources for sulfur.
- The microelements like: Fe, Mn, B, Zn, Mo, Cu, I and Co Fe is generally supplied as chelated EDTA complex.
B. Organic nutrients:
- Carbohydrates : Standard carbon source is sucrose at a concentration of 2-5 per cent.
- Vitamins: Among the vitamins only thiamine (B₁) seems to be universally required. Other vitamins are pyrodoxine (B.). nicotinic acid (B3), riboflavin (B2), ascorbic acid (vit. C), cyanocobalamin (B12).
- Phytohormones - Plant growth regulators (PGRs) like auxins, cytokinins, gibberellins, ethylene and absicissic acid.
